Journal: Critical Care and Resuscitation

Article Title: An in-vitro study to characterise analytical interference caused by sodium ascorbate with point-of-care measurement of lactate and glucose

doi: 10.1016/j.ccrj.2026.100174

Figure Lengend Snippet: Measurements for lactate and blood glucose across devices.

Article Snippet: Baseline and spiked blood samples were immediately analysed at the bedside with several POC devices, based on availability, including the RAPIDPoint® 500 Blood gas system (Siemens HealthCare, Erlangen, Germany), Epoc® Blood analysis system (Siemens HeathCare, Erlangen, Germany), Radiometer ABL800 blood gas analyser (Radiometer, Copenhagen, Denmark), StatProfile® Prime Plus blood gas analyser (Nova Biomedical, Waltham, USA), i-STAT 1® blood analyser (Abbott Laboratories, North Chicago, USA), HemoCue® Glucose 201 (HemoCue, Angelholm, Sweden), FreeStyle Optium Neo (Abbott, Illinois, USA), StatStrip Xpress® lactate system (Nova Biomedical, Waltham, USA) and the StatStrip Xpress® glucose meter (Nova Biomedical, Waltham, USA) ( ).

Techniques:

Journal: Critical Care and Resuscitation

Article Title: An in-vitro study to characterise analytical interference caused by sodium ascorbate with point-of-care measurement of lactate and glucose

doi: 10.1016/j.ccrj.2026.100174

Figure Lengend Snippet: Measured lactate at baseline and target ascorbate levels for ABL800, Prime Plus, Roche colorimetric (SA Pathology), Epoc, StatStrip Xpress, and i-STAT 1 blood analyser (in series order). Data are median [IQR].

Article Snippet: Baseline and spiked blood samples were immediately analysed at the bedside with several POC devices, based on availability, including the RAPIDPoint® 500 Blood gas system (Siemens HealthCare, Erlangen, Germany), Epoc® Blood analysis system (Siemens HeathCare, Erlangen, Germany), Radiometer ABL800 blood gas analyser (Radiometer, Copenhagen, Denmark), StatProfile® Prime Plus blood gas analyser (Nova Biomedical, Waltham, USA), i-STAT 1® blood analyser (Abbott Laboratories, North Chicago, USA), HemoCue® Glucose 201 (HemoCue, Angelholm, Sweden), FreeStyle Optium Neo (Abbott, Illinois, USA), StatStrip Xpress® lactate system (Nova Biomedical, Waltham, USA) and the StatStrip Xpress® glucose meter (Nova Biomedical, Waltham, USA) ( ).

Techniques:

Journal: Journal of Translational Autoimmunity

Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach

doi: 10.1016/j.jtauto.2025.100341

Figure Lengend Snippet: Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

Article Snippet: Membranes were blocked with 5 % non-fat milk in TBS-T and incubated overnight at 4 °C with primary antibodies against phospho-AKT (Thr308), phospho-NFκB p65 (Ser536), phospho-p38 MAPK (Thr180/Tyr182), phospho-STAT1 (Tyr701), and phospho-STAT3 (Tyr705) (Cell Signaling Technology, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Protein-Protein interactions, Expressing, Phospho-proteomics, Control

Journal: Nature Aging

Article Title: p21 + TREM2 + senescent macrophages fuel inflammaging and metabolic dysfunction-associated steatotic liver disease

doi: 10.1038/s43587-026-01101-6

Figure Lengend Snippet: a , Illustration of the in vitro model system using exogenous DNA damage with irradiation and Doxo on PBMC-derived macrophages from male and female human donors. b , Mean ± s.e. gene expression (RT–qPCR) normalized to control. n = 9 donors (mixed male and female) for control and Sen(IR) conditions. n = 8 for Sen(Doxo) conditions. P value from Tukey’s test post repeated measures ANOVA analysis. c , SDS–PAGE gels and immunostaining (western blot) for p21 and p16 in response to 10 days post DNA damage. ImageJ quantification for band intensity is shown to the right and represents relative intensity over the loading control. d , SA-β-gal images of human senescent macrophages. e , Click-iT EdU labeling of cells to assess proliferation dynamics. Axes represent the percentage of cells positive for the stain relative to the parental gate, as detected by flow cytometry. The bar plot represents the mean ± s.e. of n = 3 independent donors. P value derived from unpaired one-sided t -test. f , PCA on bulk RNA-seq samples from 10 days post DNA damage for three blood donors. g , Heatmap projection of all statistically significant DEGs compared to control conditions. h , KEGG pathway enrichment analysis for the top ten pathways downregulated in human senescent macrophages. i , KEGG pathway enrichment analysis for the top ten pathways upregulated in human senescent macrophages. j , Model illustration of running MSen score on publicly accessible single-cell dataset of human liver biopsy samples from five people with liver cirrhosis and five with a healthy liver. k , UMAP projection of all annotated CD45 + cell types and colored by human MSen Seurat score. l , UMAP projection of CD68 + cells (macrophages) in healthy and cirrhotic tissue samples. Color denotes human MSen Seurat score. m , Distribution of MSen Seurat score across resident macrophage (Kupffer cells) and non-resident monocyte and macrophage populations in healthy and cirrhotic conditions. P value represents the results of a two-sided t -test. n , Distribution of human MSen score across all CD45 + cell types in the dataset. ALD, alcohol-related liver disease; F, female; KC, Kupffer cell; IgA, immunoglobulin A; IL-C, innate lymphoid cell; M, male; MoMac, monocyte-derived macrophage; Mø, macrophage; PBC, primary biliary cholangitis; pDC, plasmacytoid dendritic cell. Panels a and j created in BioRender; Salladay-Perez, I. https://biorender.com/6umuckd (2026).

Article Snippet: Mouse BMDM-derived and human PBMC-derived macrophage cultures were lysed with RNA STAT 60 (Amsbio) and ~50 ng per μl of RNA was submitted to the UCLA’s Technology Center for Genomics and Bioinformatics (TCGB) core for library preparation and 2 × 100 paired-end sequencing.

Techniques: In Vitro, Irradiation, Derivative Assay, Gene Expression, Quantitative RT-PCR, Control, SDS Page, Immunostaining, Western Blot, Labeling, Staining, Flow Cytometry, RNA Sequencing, Single Cell